iGUIDE: an improved pipeline for analyzing CRISPR cleavage specificity

iGUIDE: an improved pipeline for analyzing CRISPR cleavage specificity

Blog Article

Abstract Genome engineering methods have advanced greatly with the development of programmable nucleases, but methods for quantifying on- and off-target cleavage sites and associated deletions remain nascent.Here, Christmas Tree we report an improvement of the GUIDE-seq method, iGUIDE, which allows filtering of mispriming events to clarify the true cleavage signal.Using iGUIDE, we specify the locations of Cas9-guided cleavage for four guide RNAs, characterize associated deletions, and show that naturally occurring background DNA double-strand breaks are associated with open chromatin, gene dense regions, and chromosomal fragile Queen Canopy Bed sites.

iGUIDE is available from https://github.com/cnobles/iGUIDE.